Oct 22, 2008 the agar disc diffusion method was used to determine the antibacterial activities of the oils. Honey is a sweet food made by bees using nectar from flowers. The inhibine number involves a scale of 1 to 5 representing sequential dilutions of honey in 5% steps, from 25% to 5%. To evaluate the antibacterial activity of the individual crude extracts and their combination, the antibacterial agar well diffusion assay was employed following the methods described by different works 1921. Figure 2 shows that the antibacterial activity of a mixture of lemon juice honey that is indicated by the zone of inhibition did not change during heating to a temperature of 100 oc. Lemon grass has low antimicrobial activity on the test pathogens as compared to tetracycline, chlorophenicol, erythromycin and ketoconazone. However, the antibacterial activity was not particularly strong against any of the bacteria tested as determined by.
The lemon peel extracts in different solvents such as ethanol, methanol and acetone were subjected to antibacterial assay. Phytochemical screening, antioxidant, antibacterial. The results of antibacterial activity are clearly showed that the ethanol extract of lemon grass has antibacterial activity against all tested organisms. Antibacterial activity of fruits against escherichia coli. These extracts produced inhibitory zones of 919 mm lemon and 811. Antibacterial activity of raw lemon and honey extracts against human enteric pathogens. Praphulla rao et al biosynthesis of silver nanoparticles using lemon extract and its antibacterial activity 168 int. In other words, the antibacterial activity remained stable by heat. To evaluate the antimicrobial activity of methanolic extract from the peel of the fruit of citrus limon familyrutaceae in. Then for each disk, we measured the diameters of zones of growth inhibition of bacterial cultures. It was cut with sterile knife before the juice was squeezed out and sieved. Pdf on nov 30, 2018, balathandayutham karthikeyan and others published antibacterial activity of lemon extract find, read and cite all the research you need on researchgate. Currently, many researchers have reported the antibacterial activity of honey and found that natural unheated honey has some broadspectrum antibacterial activity when tested against pathogenic bacteria, oral bacteria as well as food spoilage bacteria.
Extracts from lippia gracilis and xylopia sericea showed antifungal activity. Benisuef university phaculty of pharmacy microbiology department 2. The activity index values of cardamom, cloves and lemon grass against li were greater than one, while activity index values of cardamom and lemon grass were greater than one against staphylococcus aureus. The antibacterial activity of honey and lemon juice. Antibacterial activity of allium sativum against staphylococcus aureus in vitro 347 was 27% at a wave length of 580 nanometer, while the light transmittance was 100% for nutrient broth used to prepare the bacteria jassim, 2003.
Citrus fruits have a broad spectrum of biological activity including antibacterial, antifungal, antidiabetic, anticancer and antiviral activities due to. The in vitro antibacterial activity of lemon peel extract against the previously mentioned microorganisms was analyzed according to the presence or absence of inhibition zones, as depicted in tables. This is a term that has been widely used since as a measure of the antibacterial activity of honey. The antibacterial activity screening of the six citrus eos on the etecs and lactobacillus species is shown in table 1. The results of the antibacterial disc diffusion assays are summarized in table 2. However, there was no activity against yeasts and moulds. Citrus limon rutaceae, commonly known as lemon, is an important medicinal plant, used mainly for its alkaloids, which are having anticancer activities, and the antibacterial potential of the crude extracts against clinically significant bacterial strains has been reported kawaii et al. Preparaton of standard dilutions of garlic extract the dilution were prepared by using ethylene glycol which. The aim of this work was to determine the effectiveness of the essential oils eos of lemon citrus lemon l. All eo studied had some inhibitory effect on the six.
Hayes and markovic in 2002 investi gated the antimicrobial properties of lemon and found that lemon possesses significant antimicrobial activity against. Antibacterial activity of lemon, caraway and basil extracts on the growth of listeria spp. Evaluation of the antibacterial activity of citrus juices alrafidain. As microorganism are becoming resistant to present day antibiotics, our study focuses on antimicrobial activity and future prophylactic potential of the lemon peel. Jul 30, 2015 antibacterial activity of ethanolic extracts of phyllanthus amarus against extended spectrum lactamase producing escherichia coli isolated from stool samples of hiv sero.
Superior antibacterial activity of integral lemon pectin from. Backhousia citriodora products are used as bushfoods and flavorings and by the aromatherapy industry. The standardized bacterial broth culture was streaked. The bacterial inoculum was prepared and was spread with a sterile cotton swap into petri dishes 90 mm of diameter containing 20 ml of mh agar. Pectin extracted via hydrodynamic cavitation in water only from waste lemon peel and further isolated via freeze drying displays significant antibacterial activity against staphylococcus aureus, a gram positive pathogen which easily contaminates food. Antimicrobial activity of different aqueous lemon extracts. Antibacterial activity screening by disc diffusion. Pdf study antimicrobial activity of lemon citrus lemon. Antibacterial activity test of individual and combined crude extracts. The antimicrobial activity of essential oils was determined by an impedimetric method using bactometer m64 system biomerieux.
The mic of cymbopogon citratus reveals that a higher dose of the plant extract is required to bring about a significant activity in the body. Biosynthesis of silver nanoparticles using lemon extract and. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. The extract in solvent ethanol shows higher antimicrobial activity against tested microorganisms in comparison with the extracts of lemon peel in. Achillea millifolium yarrow, caryophyllus aromaticus clove, melissa offficinalis lemon balm, ocimun basilucum basil, psidium guajava guava, punica granatum pomegranate, rosmarinus officinalis. The high zone of inhibition at low concentration proved the plants to be medically useful. In vitro antibacterial activity of citrus limon peel extracts.
The extracts from clove and jambolan presented the highest activities, i. Stability study of antibacterial activity of mixed lime juice. Antimicrobial activity of backhousia citriodora lemon myrtle. The antibacterial activity of honey and lemon juice against. The antimicrobial activity was evaluated using agar well diffusion method. This study was conducted to determine the antimicrobial activity of methanol and ethanol extracts of peels of pomegranate punica grana, orange citrus siensis and lemon limona taris against four foodborne pathogens listeria monocytogenes. Acetic acid, kombucha samples and heatdenaturated kombucha showed significant antimicrobial activity against bacteria. Zone of inhibition mm against different microorganisms extracts aspergillus niger mtcc 281 candidaalbicans mtcc 3017 lemon juice 25 27. The extracts from basil, clove, guava, jambolan, lemon balm, pomegranate, rosemary and thyme presented antimicrobial activity to at least one of the tested microorganisms. Pdf antimicrobial activity of different aqueous lemon. Pdf study antimicrobial activity of lemon citrus lemon l. Fruits were collected for their bacterial activity in the summer. Citrus flavanoid has a large spectrum of biological activity including antibacterial, antifungal anti diabetic, anticancer and antiviral activities 18.
Lemon species might have antimicrobial activity against different grampositive, gramnegative and yeast pathogens and could be used for prevention of various diseases caused by these organisms. In vitro antibacterial activity of selected medicinal plants. The antibacterial value of straight chain alcohols increases with an increase in the molecular weight and beyond c8 the activity begins to fall off. In most ancient cultures honey has been used for both nutritional and medical purposes. Antibacterial activity of citrus limonum fruit juice extract. Lemon eo showed the highest inhibition effect upon s. Evaluation of antibacterial activity of lemon verbena lippiacitriodora leaves hadi koohsari 1, ezzat allah ghaemi 2, maryam sadegh shesh poli 3 and ali sadegh 1 1department of microbiology, faculty of basic sciences, islamic azad university, azadshahr branch, iran 2department of microbiology, golestan university of medical sciences, gorgan, iran. Formulation, evaluation and antibacterial efficiency of water. Sonawane department of microbiology, shivaji university.
Antimicrobial activity of cymbopogon citratus lemon grass. Antibacterial activity of different fruits this study was conducted to analyze the antibacterial activity of different eight available seasonal fruits mango, apricot, grapes, peach, apple, lemon, melon and water melon against escherichia coli. Antimicrobial activity of lavender, tea tree and lemon oils. The selected essential oils were screened against four gramnegative bacteria escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus vulgaris and two grampositive bacteria bacillus subtilis and staphylococcus aureus at four different concentrations 1. Pdf antibacterial activity of pomegranate, orange and lemon. The antibacterial activity of 14 essential oils and their major constituents in the gaseous state was evaluated against haemophilus influenzae, streptococcus pneumoniae, streptococcus pyogenes and staphylococcus aureus. Citrus flavonoids have a large spectrum of biological activity including antibacterial, antifungal, antidiabetic, anticancer and antiviral activities 7. Study antimicrobial activity of lemon citrus lemon l. The main objective of the study is extraction, identification of antimicrobial compounds and demonstration of antimicrobial activity of lemon citrus lemon l. This study justifies the use of honey and lemon juice separately and in mixture as an alternative medicine in the treatment of respiratory tract infections. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against. Antibacterial activity of citrus limonon acnevulgaris pimples. This is presumably due to the heating process in a short.
The research demonstrates that the peel of lemon is also a useful antimicrobial agent as well as an astringent 20. Antibacterial, lemon juice, citrus limonum, enthomedicine, nigeria. The agar disc diffusion method was used to determine the antibacterial activities of the oils. Unraveling the selective antibacterial activity and chemical.
In vitro antibacterial activity of some plant essential oils. All eo studied had some inhibitory effect on the six bacteria tested. This indicates that the spices specified exhibit more antimicrobial activity than the antibiotic penicillin which is the most. Strain quantity of spice extract ldisc 5 10 20 inhibition zone diameter mm lemon l. Little or no activity was found to be associated with the leaf tea and hydrosol, respectively. The extract in solvent ethanol shows higher antimicrobial activity against tested microorganisms in comparison with the extracts of lemon peel in other solvents like methanol and acetone. Table 3 24 showed the inhibition zone of ethanol extract of lemon grass by disk diffusion method as shown in table 3. This study was setup to authenticate the folklore claims regarding the antibacterial activity of c.
In this regard, the antibacterial activity of lemon juice extract against bacteria strains isolated locally from clinical specimens and typed strains from the american. Lemon and basil extracts inhibited all the organisms at the level of. Honey, lemon juice, synergistic effect, antibacterial activity, zone of. Antibacterial activity of ethanolic extracts of phyllanthus amarus against extended spectrum lactamase producing escherichia coli isolated from stool samples of hiv sero. Phytochemical, antimicrobial, and antioxidant activities of. Investigate the antimicrobial activity of raw lemon and. Antibacterial activity of plant extracts and phytochemicals. The antibacterial effect of the new integropectin is largely superior to that of commercial citrus pectin, opening the way to advanced.
Antibacterial activity of lemon juice, lemon peel extract and citric acid. Thus the above study depicts that antimicrobial activity of lemon oil is much better. The isomeric alcohol shows a drop in activity from primary, secondary to tertiary. Antibacterial activity of lemongrass cymbopogon citratus. The extract of caraway showed activity only against l. Antibacterial activity of citrus limonon acnevulgaris. In the caribbean, south america and africa, lemon juice is believed to be effective against diphtheria and upper respiratory tract infections pamplonaroger, 1999. Comparative study of antimicrobial effects of lemon oil and peel. Antifungal activity of raw lemon and honey extracts against pathogenic fungi.
To evaluate the antibacterial activity of 21 plant essential oils against six bacterial species. Investigate the antimicrobial activity of raw lemon and honey. Lemon citrus limon juice has antibacterial potential. The use of honey as a traditional remedy for microbial infections dates back to ancient times. The lemon was washed with water to remove sand and other particles and rinsed with sterile distilled water. Introduction lemon juice is believed to have antimicrobial properties in many cultures of the world. We evaluated the antimicrobial activity of extracts of citrus limon by the method of diffusion in a solid medium. Antibacterial activity of essential oils and their major. Antimicrobial and antioxidant efficacy of citrus limon l.
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